KARAKTERISASI ENZIM α- AMILASE PENGGEREK BATANG KUNING (Scirpophaga Incertulas) PADA TANAMAN PADI DI JEMBER

Abstract

Rice stem borer is one of the most common pests that attack rice plants with an attack intensity of up to 90%. This pest attacks rice plants in various stages of growth ranging from the vegetative to generative phase. The rice stem borer damages plant tissue systems by eating the stems and converting them into carbides in the form of starch from rice plants. Control of stem borer using biotechnology can be done by inhibiting the metabolic cycle of the insect. This control method is carried out by stopping the -amylase enzyme using a protein inhibitor available in rice seeds. - amylase is an enzyme that plays a role in the degradation process of starch into simpler forms, both in microorganisms, plants and humans. The research was conducted at the Agrotechnology laboratory, Jember University. The research began with the isolation and purification of the -amylase enzyme from the brown planthopper and then continued with testing the -amylase activity using the Bernfeld method. The test parameters include the effect of temperature, pH, and substrate concentration on the activity of the -amylase enzyme.